| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-ULK1 (S556) was used as the immunogen for the Phospho-ULK1 (Ser556) antibody. |
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Overview
Phospho-ULK1 (Ser556) Antibody / Unc-51 like autophagy activating kinase 1 is a anti-ULK1 (phospho-Ser556) Rabbit antibody Recombinant Rabbit Monoclonal clone 31U30 supplied in Liquid format. Recommended for workflows such as Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: ULK1 (phospho site: Ser556)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31U30, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): IF, ICC, WB
Biological background
ULK1 functions as the mammalian homolog of yeast Atg1, acting at the top of the autophagy hierarchy. Research using Phospho-ULK1 (Ser556) antibody has demonstrated that phosphorylation at serine 556 by AMP activated protein kinase promotes activation of ULK1 under conditions of energy depletion. This modification stimulates autophagy initiation by enabling the ULK1 complex to recruit downstream autophagy machinery. By contrast, mTORC1 signaling suppresses ULK1 through phosphorylation at other sites, highlighting the dynamic control of ULK1 activity by nutrient and energy signals.
Phospho-ULK1 (Ser556) antibody has been used to show that phosphorylation at this site acts as a molecular switch for autophagy induction. In starvation or low energy states, phosphorylation at Ser556 ensures cells adapt by recycling cytoplasmic contents through lysosomal degradation. This process provides metabolic substrates for survival and maintains homeostasis. Inhibiting phosphorylation at Ser556 impairs autophagy, underscoring its essential role in this pathway.
Dysregulation of ULK1 phosphorylation has been linked to human disease. Studies with Phospho-ULK1 (Ser556) antibody have revealed that impaired autophagy contributes to neurodegenerative diseases, metabolic disorders, and cancer. In cancer, altered ULK1 phosphorylation influences tumor cell survival under stress conditions, promoting growth in nutrient-poor environments. In neurodegeneration, reduced ULK1 activation leads to accumulation of toxic proteins. These findings emphasize the clinical importance of monitoring ULK1 phosphorylation states.
Phospho-ULK1 (Ser556) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals phosphorylation-dependent shifts in ULK1 activity, immunohistochemistry highlights tissue-specific patterns of autophagy activation, and immunofluorescence demonstrates dynamic localization of phosphorylated ULK1 at autophagosome initiation sites. These applications make Phospho-ULK1 (Ser556) antibody an indispensable reagent in autophagy research.
By supplying validated Phospho-ULK1 (Ser556) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
