| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-Vimentin (S39) was used as the immunogen for the Phospho-Vimentin (Ser39) antibody. |
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| Product Type | |
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Overview
Phospho-Vimentin (Ser39) Antibody / VIM is a anti-VIM (phospho-Ser39) Rabbit antibody Recombinant Rabbit Monoclonal clone 31V35 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: VIM (phospho site: Ser39)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31V35, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-Vimentin (Ser39) antibody is widely applied in cancer research, cell biology, and developmental studies. Vimentin phosphorylation at Ser39 facilitates cytoskeletal reorganization, promoting epithelial to mesenchymal transition, migration, and invasion. In cancer, elevated phospho vimentin correlates with metastasis and poor prognosis. By detecting phosphorylation at Ser39, researchers can investigate how cytoskeletal remodeling contributes to tumor progression and cell plasticity.
Western blot assays reveal phosphorylated vimentin bands distinct from total protein. Immunohistochemistry highlights phospho vimentin in invasive tumor regions, while immunofluorescence maps cytoplasmic distribution in migrating cells. These applications make Phospho-Vimentin (Ser39) antibody valuable for tracking cytoskeletal changes in disease and development.
Phosphorylation of vimentin integrates mechanical and chemical signals that regulate mitosis, adhesion, and stress adaptation. By applying Phospho-Vimentin (Ser39) antibody, scientists can evaluate how phosphorylation controls filament turnover and supports cellular responses to growth factors and mechanical stress. Its use in combination with total vimentin antibody provides a complete picture of cytoskeletal regulation.
Beyond cancer, phospho vimentin is important in wound healing, embryogenesis, and inflammation. Dynamic filament remodeling ensures proper migration of fibroblasts, endothelial cells, and immune cells during tissue repair and development. The phospho-specific antibody therefore provides a versatile tool for exploring structural regulation in multiple biological systems.
Phospho-Vimentin (Ser39) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
