| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human VPS34/PIK3C3 was used as the immunogen for the PIK3C3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PIK3C3 Antibody / VPS34 / Phosphatidylinositol 3-kinase catalytic subunit type 3 is a anti-PIK3C3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PIK3C3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Functionally, PIK3C3 antibody recognizes a 887-amino-acid cytoplasmic protein that catalyzes the phosphorylation of phosphatidylinositol to generate PI3P, recruiting downstream effectors such as WIPI and DFCP1. PIK3C3 forms multiprotein complexes with Beclin 1, UVRAG, ATG14, and VPS15 to regulate membrane nucleation during autophagy and endosome maturation. Its lipid kinase activity is crucial for endocytic trafficking, nutrient sensing, and organelle biogenesis.
The PIK3C3 gene, located on chromosome 18q12.3, is ubiquitously expressed and evolutionarily conserved from yeast to humans. It governs autophagic flux by mediating vesicle formation and fusion with lysosomes. Through its interaction with Beclin 1, PIK3C3 serves as a core regulator of cellular homeostasis under nutrient stress and is involved in tumor suppression and neuroprotection.
Pathologically, dysregulation of PIK3C3/VPS34 contributes to cancer, neurodegeneration, and infectious disease. Inhibition of VPS34 impairs autophagy, leading to protein aggregation and metabolic imbalance. Conversely, enhanced PIK3C3 activity supports tumor cell survival under nutrient deprivation. The enzyme is also hijacked by viruses to generate replication compartments. Research using PIK3C3 antibody advances understanding of autophagic control, endosomal transport, and signaling adaptation to stress.
PIK3C3 antibody is suitable for western blotting, immunofluorescence, and immunoprecipitation to detect VPS34 and its complexes. It supports studies in autophagy, intracellular trafficking, and lipid signaling.
Structurally, PIK3C3 consists of a helical domain, a C2 domain for membrane association, and a lipid kinase catalytic domain. Its activity depends on interactions with VPS15 and Beclin 1 for stabilization and subcellular localization. This antibody facilitates analysis of PIK3C3/VPS34's central role in autophagy initiation and endolysosomal trafficking.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
