| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human PI3 Kinase p110 beta was used as the immunogen for the PIK3CB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
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Overview
PIK3CB Antibody / PI3 Kinase p110 beta is a anti-PIK3CB Rabbit antibody Recombinant Rabbit Monoclonal clone 30P53 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PIK3CB
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P53, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
PIK3CB antibody is widely used in cancer biology, immunology, and metabolic research. PI3 kinase p110 beta is activated downstream of receptor tyrosine kinases, G protein coupled receptors, and integrins, playing a unique role in signaling integration. Dysregulation of PI3 kinase activity contributes to oncogenesis, insulin resistance, and immune dysfunction. By detecting the beta catalytic subunit, researchers can investigate isoform specific functions that differ from those of PI3 kinase alpha, delta, or gamma.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, PIK3CB antibody identifies the protein with high specificity, enabling quantitative comparisons across cell types and conditions. Immunohistochemistry localizes expression patterns in tissues, with high levels often detected in tumors. Immunofluorescence studies reveal subcellular localization at the plasma membrane and cytosol, consistent with its role in lipid signaling.
PI3 kinase p110 beta has drawn particular attention in oncology. It is frequently upregulated in prostate and breast cancers, and mutations or amplification of PIK3CB drive abnormal cell survival signaling. Targeted inhibition of p110 beta is under active investigation as a therapeutic approach, particularly for tumors resistant to inhibitors of other PI3 kinase isoforms. Monitoring expression with PIK3CB antibody helps define the contribution of this isoform to tumor biology and drug sensitivity.
Beyond oncology, PIK3CB antibody is relevant to immunology. PI3 kinase beta regulates platelet activation, neutrophil migration, and T cell signaling. Altered activity contributes to inflammation and autoimmunity, making it an important target for therapeutic modulation. In metabolism research, it plays a role in insulin signaling and glucose homeostasis, with implications for diabetes and metabolic syndrome.
PIK3CB antibody offered by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
