| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human POLD1 recombinant protein (Position: D25-D1068) was used as the immunogen for the POLD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLD1 Antibody / DNA polymerase delta catalytic subunit is a anti-POLD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: POLD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, ELISA
Biological background
Structurally, POLD1 is a large enzyme of approximately 125 kilodaltons containing conserved polymerase motifs and an N-terminal exonuclease domain. It forms a heterotetrameric complex with POLD2, POLD3, and POLD4 subunits, supported by interactions with PCNA (proliferating cell nuclear antigen) and replication factor C. This complex coordinates DNA elongation, repair synthesis, and recombination processes, playing a vital role in cell cycle progression and genome maintenance.
The POLD1 antibody is widely used in molecular biology, cancer research, and DNA repair studies to investigate replication fidelity, mutagenesis, and polymerase function. Western blot analysis detects a 125 kilodalton band corresponding to the catalytic subunit, while immunofluorescence shows nuclear localization during S phase and DNA damage repair. This antibody provides a robust tool for assessing DNA replication machinery and the maintenance of genomic integrity.
Mutations in POLD1 cause Polymerase Proofreading-Associated Polyposis and predispose to colorectal and endometrial cancers due to defective proofreading activity and increased mutational load. Additionally, POLD1 variants are linked to syndromes involving premature aging and mitochondrial dysfunction. Its role extends beyond replication to DNA damage tolerance and translesion synthesis. The POLD1 antibody supports exploration of polymerase fidelity mechanisms, DNA repair pathways, and tumor suppressor networks involving replication stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
