| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A peptide sequence specific to DNA polymerase subunit gamma 1 was used as the immunogen for the POLG antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLG Antibody / DNA polymerase subunit gamma 1 is a anti-POLG Rabbit antibody Recombinant Rabbit Monoclonal clone 24P93 supplied in Purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: POLG
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 24P93, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB
Biological background
Mutations in POLG are associated with a range of mitochondrial disorders, including progressive external ophthalmoplegia, Alpers-Huttenlocher syndrome, and ataxia-neuropathy syndromes. Studying POLG function helps researchers understand mitochondrial genome maintenance and the molecular basis of mitochondrial diseases.
Using a high-quality POLG antibody allows for sensitive detection of the protein in assays such as western blot, immunohistochemistry, and immunofluorescence. A POLG antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
