| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human POLG2 recombinant protein (Position: Q29-E434) was used as the immunogen for the POLG2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLG2 Antibody / DNA polymerase subunit gamma-2 is a anti-POLG2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: POLG2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
POLG2 is encoded by the POLG2 gene located on human chromosome 17q23.3. The protein is approximately 485 amino acids in length and localizes to the mitochondrial matrix. It forms a homodimer that binds to POLG, increasing its affinity for DNA and reducing dissociation during replication. Through this interaction, POLG2 coordinates mtDNA replication and repair under both physiological and stress conditions.
The POLG2 antibody detects a 55 kilodalton band by western blot and shows mitochondrial punctate staining under immunofluorescence microscopy. Loss-of-function mutations in POLG2 result in reduced mtDNA copy number, leading to multiple mitochondrial DNA deletions, oxidative stress, and mitochondrial depletion syndromes. Clinical manifestations include progressive external ophthalmoplegia, ataxia, and myopathy.
POLG2 also contributes to mitochondrial biogenesis and interacts with nucleoid proteins such as TFAM and TWINKLE, supporting DNA packaging and maintenance. Dysregulation of POLG2 expression has been observed in neurodegenerative diseases, aging tissues, and metabolic disorders, where impaired mtDNA replication leads to reduced energy output and increased reactive oxygen species production.
Because of its essential role in maintaining mitochondrial genome integrity, POLG2 serves as a critical factor in understanding mitochondrial pathophysiology and age-related dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
