Poly-L-Lysine Plates

Poly-L-lysine Plates utilize their high density of cationic groups to form strong electrostatic and stereospecific bonds with negatively charged molecules. The surface is created using a synthetic molecule rich in α-amino, α-carboxyl, and ε-amino groups, while preserving the polystyrene’s optical properties, making it a versatile tool for a wide range of biological assays. The Poly-L-lysine Coated Plates plates are particularly well-suited for cell adhesion, as they provide an ideal environment for cells to attach due to their positive charge, which interacts with the negatively charged cell membrane. Key applications include interactions with plasminogen and plasminogen activators, ribosoma

Poly-L-lysine Platesare pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Key Features

• Poly-L-lysine surface: It is particularly well-suited for cell adhesion, as it provides an ideal environment for cells to attach due to their positive charge, which interacts with the negatively charged cell membrane.
• It interacts with plasminogen and plasminogen activator
• It interacts with ribosomal RNA
• It interacts with double stranded DNA
• Blocking: Plates are pre blocked with non proteic blocking buffer (BlockerWell)
• Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
• Wells design: flat bottom.
• Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
• Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
  • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
• Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
• Alphanumeric Coding: For easy and accurate well identification during experiments.
• Recommended working volume: of 75 to 200 μl
• Packaging: Each coated plate is packed in a single barrier bag with desiccant
• Unit: Contains 5 plates
• Minimum order: 10 plates.
• Ready to use

Key Benefits

• Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
  • The mould design provides optical quality, important to reduce the background signal.
  • The rim protects the external face of the bottom from scratches
• Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
• Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
• Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
• Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Applications

• ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
• Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
• Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
• Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.