| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids M1-I241 from the human protein were used as the immunogen for the PSMA5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMA5 Antibody / Proteasome subunit alpha type 5 is a anti-PSMA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA, Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat, Monkey. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMA5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P, FACS, ELISA, IF
Biological background
PSMA5 contributes to the assembly and stability of the proteasome complex. The alpha subunits, including PSMA5, form a heptameric ring that shapes one end of the barrel-like core. This arrangement functions as a regulated gate, opening to allow access of protein substrates targeted for degradation. By influencing the selectivity and efficiency of protein turnover, PSMA5 is critical for cellular responses to stress, metabolic regulation, and removal of damaged or misfolded proteins.
Research into PSMA5 has highlighted its importance in diverse biological processes. Proteasome activity, dependent on components such as PSMA5, is required for antigen processing, generating peptides that are presented by MHC class I molecules to cytotoxic T lymphocytes. This immune surveillance mechanism is essential for identifying and destroying infected or transformed cells. In addition, proteasome function regulates transcription factors, signaling proteins, and cell cycle regulators, placing PSMA5 at the heart of pathways that determine proliferation, differentiation, and apoptosis.
Alterations in proteasome activity have been associated with cancer, neurodegenerative disorders, and inflammatory disease. Dysregulation of PSMA5 expression or proteasome activity can disrupt protein turnover, contributing to abnormal signaling and accumulation of toxic protein aggregates. Pharmacologic inhibitors of the proteasome, such as bortezomib, underscore the therapeutic relevance of targeting this pathway, with PSMA5 as a structural and functional element within the proteasome complex.
The PSMA5 antibody is widely used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect expression levels and subcellular localization of this proteasome subunit. Such applications are essential for assessing changes in protein degradation capacity across tissues, during disease progression, or under experimental manipulation. For scientists investigating protein quality control, immune responses, or therapeutic intervention strategies, the PSMA5 antibody provides a reliable and precise detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
