| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PSMA6 recombinant protein (Position: M1-D246) was used as the immunogen for the PSMA6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMA6 Antibody / Proteasome subunit alpha type 6 is a anti-PSMA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMA6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
PSMA6 is encoded by the PSMA6 gene located on human chromosome 14q13.2. The protein is approximately 27 kilodaltons and forms one of seven alpha subunits that compose the outer rings of the cylindrical 20S core particle. Together with beta subunits, these structures assemble into the functional 26S proteasome when associated with regulatory particles such as PA700 or PA28. PSMA6 is critical for maintaining proteolytic efficiency and specificity by regulating substrate entry and gate opening.
The PSMA6 antibody detects a 27 kilodalton protein by western blot and reveals strong cytoplasmic and nuclear staining, consistent with proteasome distribution. PSMA6 contributes to the degradation of short-lived proteins involved in cell cycle progression, apoptosis, and signal transduction. Through the ubiquitin-proteasome system (UPS), PSMA6 supports protein quality control and adaptive responses to oxidative or metabolic stress.
Mutations or altered expression of PSMA6 have been linked to cardiovascular and autoimmune diseases, as well as cancer. Overexpression in tumors enhances proteasomal activity and supports rapid cell proliferation, while reduced expression leads to accumulation of damaged proteins and cellular dysfunction. In immune cells, PSMA6 influences antigen processing and presentation, shaping adaptive immune responses.
Because of its fundamental role in protein degradation, PSMA6 serves as a biomarker for proteasome activity and a potential target for therapeutic intervention in cancer and neurodegenerative diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
