| Field | Specification |
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| Immunogen | A synthesized peptide derived from human PSMB10 was used as the immunogen for the PSMB10 antibody. |
| Isotype | |
| Product Type | |
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| Reactivity | |
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| Target | |
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Overview
PSMB10 Antibody / Proteasome subunit beta type 10 is a anti-PSMB10 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P25 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PSMB10
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P25, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
The proteasome is a multicatalytic protease complex responsible for degrading ubiquitinated proteins. Research using PSMB10 antibody has demonstrated that incorporation of Proteasome subunit beta type-10 into the 20S proteasome replaces the standard catalytic subunit beta type-2, conferring altered cleavage specificity optimized for generating peptides that bind MHC class I molecules. This substitution enhances immune surveillance by producing peptides with anchor residues suited for antigen presentation.
PSMB10 is inducible by interferon gamma, which promotes immunoproteasome assembly in response to infection. Studies with PSMB10 antibody have shown that upregulation of this subunit increases antigenic peptide supply, facilitating recognition of infected or malignant cells by cytotoxic T lymphocytes. This mechanism highlights the link between interferon signaling, proteasome remodeling, and adaptive immunity.
Beyond its role in immunity, altered expression of Proteasome subunit beta type-10 has been implicated in disease. Research using PSMB10 antibody has revealed that dysregulation of immunoproteasome activity contributes to autoimmunity, cancer, and neurodegeneration. Overactive immunoproteasome function can enhance self-antigen presentation, driving autoimmunity, while impaired activity reduces antigen presentation and immune defense. These findings make PSMB10 a critical focus in both basic and translational research.
PSMB10 antibody is applied in western blotting, immunohistochemistry, and flow cytometry. Western blotting confirms immunoproteasome incorporation, immunohistochemistry highlights tissue-specific expression, and flow cytometry quantifies levels in immune cells. These applications make PSMB10 antibody essential in immunology and proteostasis research.
By supplying validated PSMB10 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
