| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids K21-L440 from the human protein were used as the immunogen for the PSMC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMC1 Antibody / 26S Proteasome regulatory subunit 4 is a anti-PSMC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA, Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMC1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P, FACS, ELISA, IF
Biological background
PSMC1, also known as Rpt2, is one of six ATPases that form a heterohexameric ring in the base of the 19S regulatory particle. Through ATP hydrolysis, PSMC1 contributes to unfolding ubiquitinated substrates and translocating them into the 20S catalytic chamber for degradation. This activity is essential for controlling protein quality, regulating the cell cycle, modulating transcription factor activity, and coordinating stress responses. By ensuring efficient substrate processing, PSMC1 plays a fundamental role in preserving cellular function under both normal and stress conditions.
The proteasome system, which depends on subunits such as PSMC1, regulates diverse pathways including antigen processing for MHC class I presentation. This immune-related function is critical for defense against pathogens and recognition of abnormal cells. In addition, proteasome-mediated degradation influences signaling cascades involving NF-kB, p53, and cyclins, linking PSMC1 directly to processes such as apoptosis, proliferation, and DNA repair.
Altered proteasome activity has been associated with cancer, neurodegenerative disorders, and autoimmune disease. Changes in expression or activity of PSMC1 may disrupt protein turnover, contributing to accumulation of toxic protein aggregates or dysregulated signaling. As proteasome inhibitors have already proven valuable in cancer therapy, individual components like PSMC1 are of increasing interest for their roles in disease biology and therapeutic targeting.
The PSMC1 antibody is widely applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to study protein expression, localization, and regulation. These applications allow researchers to investigate proteasome function in health and disease, assess subunit composition, and explore the effects of genetic or pharmacologic manipulation. For investigators examining protein quality control, immune surveillance, or therapeutic mechanisms, the PSMC1 antibody offers a reliable detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
