| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PSMC5 was used as the immunogen for the PSMC5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMC5 Antibody / 26S proteasome regulatory subunit 8 is a anti-PSMC5 Rabbit antibody Recombinant Rabbit Monoclonal clone 29P84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PSMC5
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29P84, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
The 26S proteasome is responsible for the majority of regulated intracellular protein degradation in eukaryotic cells. By removing misfolded proteins, damaged proteins, and short lived regulatory proteins, the proteasome maintains proteostasis and controls key signaling events. Research using PSMC5 antibody has shown that 26S proteasome regulatory subunit 8 contributes to ATP driven unfolding, ensuring substrate entry into the proteolytic chamber. This function is essential for turnover of cell cycle regulators, transcription factors, and signal transduction intermediates.
Dysfunction of the proteasome contributes to numerous diseases, including neurodegenerative disorders such as Parkinson and Alzheimer disease, where accumulation of protein aggregates is a hallmark. Research with PSMC5 antibody has demonstrated that altered proteasome activity leads to impaired clearance of aggregation prone proteins. In cancer, hyperactivation of the proteasome allows degradation of tumor suppressors and cell cycle inhibitors, promoting unchecked proliferation. PSMC5 has also been implicated in immune regulation, where proteasomal processing generates antigenic peptides presented on MHC class I molecules.
PSMC5 antibody is widely used in western blotting, immunoprecipitation, and immunohistochemistry. Western blotting demonstrates abundance in metabolically active tissues, while immunoprecipitation allows isolation of 19S regulatory complexes containing PSMC5. Immunohistochemistry highlights proteasome distribution in brain, muscle, and immune organs. These applications make PSMC5 antibody versatile in both fundamental and translational research.
By supplying validated PSMC5 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
