| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PSMD5 recombinant protein (Position: M1-K493) was used as the immunogen for the PSMD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMD5 Antibody / 26S proteasome non-ATPase regulatory subunit 5 is a anti-PSMD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PSMD5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Functionally, PSMD5 antibody identifies a 328-amino-acid cytoplasmic protein that binds proteasome regulatory subunits and interferes with their association into the mature 26S proteasome. PSMD5 fine-tunes proteasome activity by limiting the assembly of regulatory particles with the catalytic core, maintaining balance between protein degradation and stability. This regulation is crucial for cell cycle control, stress response, and antigen presentation.
The PSMD5 gene is located on chromosome 9q33.3 and is expressed in many tissues, including heart, brain, and skeletal muscle. PSMD5 expression is induced by stress and inflammatory signals to transiently reduce proteasome activity, preventing excessive protein turnover during cellular adaptation.
Pathologically, PSMD5 dysfunction affects protein quality control and contributes to neurodegenerative diseases, inflammation, and cancer. Overexpression inhibits proteasome activity, leading to accumulation of misfolded proteins, while deficiency may accelerate degradation of key signaling molecules. Research using PSMD5 antibody supports studies in proteostasis, protein degradation, and stress response pathways.
PSMD5 antibody is validated for western blotting, immunofluorescence, and immunoprecipitation to detect proteasome regulatory subunits.
Structurally, 26S proteasome non-ATPase regulatory subunit 5 contains a conserved PI31 domain responsible for proteasome binding and inhibitory function. This domain interacts with the base subcomplex of the regulatory particle, competing with normal assembly factors. This antibody enables detailed study of PSMD5's role in proteasome regulation and protein degradation dynamics.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
