| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PSME3 recombinant protein (Position: 3-251) was used as the immunogen for the PSME3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSME3 Antibody / Proteasome activator complex subunit 3 is a anti-PSME3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, possible cytoplasm.
Key elements and design rationale
- Target: PSME3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, ELISA
Biological background
PSME3 forms homoheptameric rings that cap the 20S proteasome, enhancing peptide entry and release. This activation facilitates the degradation of short-lived or misfolded proteins without requiring ubiquitination or ATP. The protein is dynamically regulated through phosphorylation and interaction with other nuclear components, enabling rapid responses to cell stress or metabolic shifts. Key substrates of PSME3 include p21, p53, and steroid receptor coactivators, indicating its dual role in proteostasis and transcriptional regulation.
The PSME3 antibody is widely used in cell biology, oncology, and proteasome regulation research to assess nuclear proteasome activity and protein turnover. Western blot analysis identifies a 29 kilodalton band corresponding to PA28 gamma, and immunofluorescence reveals diffuse nuclear staining. This antibody supports studies of proteolytic regulation in proliferation, apoptosis, and DNA damage responses. Because PSME3 controls the degradation of regulatory proteins involved in the cell cycle and tumor suppression, its dysfunction is associated with oncogenesis and altered stress tolerance.
In cancer, overexpression of PSME3 promotes tumor progression by accelerating degradation of p53 and p21, thereby weakening cell-cycle checkpoints and apoptosis. Conversely, loss of PSME3 function leads to accumulation of damaged proteins and proteostasis stress. Beyond cancer, PSME3 contributes to chromatin regulation, immune surveillance, and viral infection control. The PSME3 antibody enables researchers to examine these diverse processes by providing reliable detection in western blot, immunofluorescence, and immunohistochemistry.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
