| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PSME4 recombinant protein (Position: Q391-L657) was used as the immunogen for the PSME4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSME4 Antibody / Proteasome activator complex subunit 4 is a anti-PSME4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: PSME4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
PSME4 is encoded by the PSME4 gene located on human chromosome 2q37.1. The protein is approximately 1,840 amino acids long and forms a ring-like structure that caps one end of the 20S proteasome. PSME4-mediated activation alters proteasome activity toward oxidized and acetylated substrates, enhancing degradation of histones and DNA repair factors following genotoxic stress.
The PSME4 antibody detects a 220 kilodalton protein by western blot and shows strong nuclear localization under immunofluorescence microscopy. PSME4 plays a crucial role in DNA double-strand break repair by facilitating turnover of repair-associated chromatin proteins, including histones and Ku70/80. In male germ cells, PSME4 regulates histone replacement during spermatogenesis, ensuring chromatin condensation and sperm maturation.
Deficiency of PSME4 leads to impaired proteasome activation and accumulation of damaged proteins, resulting in genomic instability and infertility. PSME4 also influences transcriptional regulation and aging by modulating histone acetylation and chromatin dynamics. In cancer, aberrant PSME4 expression alters proteasome activity, contributing to resistance against apoptosis and therapeutic stress.
As a specialized proteasome activator, PSME4 provides insight into the noncanonical regulation of proteolysis and DNA repair.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
