| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PTEN was used as the immunogen for the PTEN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PTEN Antibody / Phosphatase and tensin is a anti-PTEN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PTEN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Phosphatase and tensin homolog acts at the plasma membrane to regulate signal transduction and control downstream kinases that drive proliferation and survival. Loss of PTEN function results in constitutive activation of AKT and mTOR pathways, leading to uncontrolled cell growth and oncogenesis. The PTEN antibody allows visualization of protein expression across tissues, facilitating analysis of its localization in both cytoplasmic and nuclear compartments. Nuclear PTEN has additional roles in maintaining chromosomal stability and DNA repair, expanding its tumor-suppressive functions beyond lipid dephosphorylation.
Mutations or deletions in the PTEN gene occur in many cancers, including glioblastoma, prostate, breast, and endometrial carcinomas. Germline PTEN mutations cause PTEN hamartoma tumor syndromes such as Cowden syndrome, characterized by benign and malignant tumor development. The PTEN antibody is widely used in diagnostic and research settings to assess expression loss and mutation effects in tumor samples. Detection of reduced PTEN levels by immunohistochemistry is an important indicator of dysregulated PI3K signaling and poor prognosis.
In addition to tumor suppression, Phosphatase and tensin homolog regulates cellular processes such as migration, apoptosis, and metabolism. It influences insulin signaling, oxidative stress response, and stem cell renewal. The PTEN antibody supports mechanistic studies into these diverse biological roles and enables quantification of protein abundance in cultured cells and tissue models. By revealing PTEN localization and activation states, this antibody provides insight into how phosphorylation, ubiquitination, or oxidation modulate its function under physiological and stress conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
