QuantiChrom™ Whole Blood Hb Kit

Overview

For quantitative determination of hemoglobin (Hb) in whole blood. The assay uses OD570nm for signal readout. Compatible sample input includes Whole blood samples. Typical stated assay timing is 5 min.

Key elements and design rationale

• Readout format: OD570nm supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Whole blood samples, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 0.2 g/dL for interpreting low-signal samples.
• Feature emphasis: Safety. Non-hazardous. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin’s reagent.

Additional feature notes highlight Simple and high-throughput. The “mix-and-read” procedure involves the addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day; Versatility. Assays can be executed in 96-well plates or cuvettes. Available format information for this listing includes 250 Tests.

Biological background

This product is centered on measurement of whole blood hb within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia, and dehydration. Simple, direct, and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ Whole Blood Hb kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 570 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Detection method

Colorimetric (OD 570 nm).

Detection limit and analytical sensitivity

Reported detection limit: 0.2 g/dL.

Procedures and timing

Stated procedure or timing information: 5 min.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
• Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.

Common research applications

• Prepare control-treated samples for paired use with compatible assay-kit plates.
• Benchmark assay response against defined control conditions in replicate wells.
• Optimize reagent concentration and incubation windows for plate-based comparisons.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.