| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RAD54L2 recombinant protein (Position: R144-H1037) was used as the immunogen for the RAD54L2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RAD54L2 Antibody / RAD54-like 2 is a anti-RAD54L2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Speckled nuclear.
Key elements and design rationale
- Target: RAD54L2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, RAD54L2 antibody identifies a 1330-amino-acid protein characterized by helicase-like ATPase domains and DNA-binding motifs. RAD54L2 participates in homologous recombination repair, facilitating the remodeling of chromatin to enable access of repair enzymes to damaged DNA. It also interacts with transcriptional co-regulators and nuclear hormone receptors, integrating DNA repair with transcriptional control. Through ATP hydrolysis, RAD54L2 alters nucleosome structure, promoting chromatin relaxation at sites of DNA damage.
The RAD54L2 gene is located on chromosome 3p21.31 and is widely expressed in proliferative tissues such as testis, ovary, and thymus. RAD54L2 shares structural homology with RAD54L, another member of the SWI2/SNF2 family involved in recombination repair, but exhibits broader functional diversity. RAD54L2 binds both double-stranded DNA and repair intermediates, enhancing strand invasion and exchange during homologous recombination. This role ensures error-free repair of double-strand breaks and prevents chromosomal rearrangements.
Beyond its DNA repair function, RAD54L2 acts as a transcriptional co-regulator by binding to androgen receptor (AR) and other nuclear receptors, modulating gene transcription in response to hormonal signaling. It is also implicated in chromatin condensation and mitotic progression. Dysregulation of RAD54L2 expression has been associated with genomic instability, altered hormone signaling, and cancer development. Elevated expression has been observed in certain tumors, where it may support DNA repair efficiency and resistance to genotoxic stress.
RAD54L2 antibody is widely used in DNA repair, chromatin remodeling, and cancer research. It is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect RAD54L2 protein localization and expression. This antibody supports studies of homologous recombination, transcriptional regulation, and genome stability. In oncology, RAD54L2 detection aids in evaluating repair pathway activation and therapeutic response to DNA-damaging agents.
Structurally, RAD54L2 contains conserved ATP-binding Walker A and B motifs and DNA-binding helicase domains characteristic of SWI2/SNF2 family proteins. Its activity is regulated by post-translational modifications and interactions with transcriptional complexes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
