Used up within 12h.<div class="

>Used up within 12h.<div class="

Supplied by Verified

View supplier profile

Fine Test · Verified supplier

Rat IL-6 (Interleukin-6) ELISA Kit

Q: The ELISA plate follows the standard size of microplate: 127.64 mm x 85.60 mm x 14.22 mm(L x W x H).<div class="

>The ELISA plate follows the standard size of microplate: 127.64 mm x 85.60 mm x 14.22 mm(L x W x H).<div class="

Q: Valid for 12 months since the production date. For the shelf life of specific batch number, please check the label printed on the kit. Before delivery, all FineTest? ELISA Kits have been subject to strict quality test.<div class="

>Valid for 12 months since the production date. For the shelf life of specific batch number, please check the label printed on the kit. Before delivery, all FineTest? ELISA Kits have been subject to strict quality test.<div class="

Q: These information is proprietary. Please contact us to learn more about clonality (polyclonality or monoclonality) and host species.<div class="

>These information is proprietary. Please contact us to learn more about clonality (polyclonality or monoclonality) and host species.<div class="

Q: Not suggested. ELISA reagents are optimized for specific batch.<div class="

>Not suggested. ELISA reagents are optimized for specific batch.<div class="

Q: Yes. The ELISA plate is dismounted. Enough component volumes are offered by 96T ELISA kit, supporting two groups of standard curve.<div class="

>Yes. The ELISA plate is dismounted. Enough component volumes are offered by 96T ELISA kit, supporting two groups of standard curve.<div class="

Q: Used up within 12h.<div class="

>Used up within 12h.<div class="

Q: FineTest? can't support validation of standard concentration outside of standard curve. Ranges of standard curve have been validated among many batches and experimenters, showing stable and accurate performance. The lowest standard concentration is the minimized range for reliable detection results. Adding higher or lower concentration of standard may cause inconsistent signal or false positive.<div class="

>FineTest? can't support validation of standard concentration outside of standard curve. Ranges of standard curve have been validated among many batches and experimenters, showing stable and accurate performance. The lowest standard concentration is the minimized range for reliable detection results. Adding higher or lower concentration of standard may cause inconsistent signal or false positive.<div class="

Q: Matrix components in serum/plasma can affect detection results. Blocking components in sample dilution buffer can decrease or remove the interference. The dilution can reduce the matrix difference between sample and standard to get better accuracy.<div class="

>Matrix components in serum/plasma can affect detection results. Blocking components in sample dilution buffer can decrease or remove the interference. The dilution can reduce the matrix difference between sample and standard to get better accuracy.<div class="

Q: FineTest? can't determine the half-life of protein in the sample(e.g. serum, plasma or cell culture supernatant). Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container. Avoid freeze-thaw cycle to prevent protein degradation.<div class="

>FineTest? can't determine the half-life of protein in the sample(e.g. serum, plasma or cell culture supernatant). Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container. Avoid freeze-thaw cycle to prevent protein degradation.<div class="

Q: Due to the specificity of each sample, it's hard to forecast and depend on sample preparation as well as analytical characteristics. Please contact us to get detection data for reference.

>Due to the specificity of each sample, it's hard to forecast and depend on sample preparation as well as analytical characteristics. Please contact us to get detection data for reference.

SKU BHE10800208 · Cat# ER0042

In Stock at Manufacturer

Quantitative double-antibody sandwich ELISA kit for measuring rat IL-6 (Interleukin-6) in Serum, Plasma, Cell Culture Supernatant, and cell or tissue lysate. Includes sensitivity 37.5pg/mL, detection range 62.5–4000pg/mL for immunology research. Includes assay time 4 hours.

At a glance

Target

Interleukin-6

Species

Rat

Sample Type(s)

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Assay Type

Sandwich ELISA, Double Antibody

Sensitivity

37.5pg/ml

Detection Range

62.5-4000pg/ml

Assay Time

4 hours

Order this product · 1 option

Catalog No.	Size	Availability	Price	Qty	Order
ER0042-96T	96 T	In Stock at Manufacturer	$455.00		Quote

Cold-chain shipping — ice packs or dry ice as required 2-8 °C for 12 months Shipping calculated at checkout · card orders +3% · Sales terms & conditions — please review before ordering. For Research Use Only. Not for diagnostic or therapeutic use.

Questions? Talk to us 1-866-986-9598 orders@biohippo.com

Overview

What is rat il-6 (interleukin-6) elisa kit?

Background

rat IL-6 (Interleukin-6) is a molecular target commonly studied in immunology, cardiovascular, and cancer research. Cytokines are secreted signaling proteins that coordinate immune responses and inflammation through receptor-mediated pathways.

Biological role and mechanism

The biological role of IL-6 is typically understood in terms of its molecular category and interaction network. Depending on the model system, it may participate in cell–cell communication, intracellular signaling, enzymatic processing, or regulation of gene expression programs. Mechanistic interpretation is often strengthened by considering upstream regulators and downstream readouts rather than relying on a single marker.

Expression and abundance of IL-6 can vary by tissue, cell type, and physiological state. In many systems, levels are influenced by factors such as developmental stage, immune activation, metabolic status, and cellular stress. Because sample matrix and pre-analytical handling can affect measured concentrations, interpretation is typically strongest when experiments keep collection and processing consistent across groups.

Nomenclature and related terms

IL-6 (Interleukin-6) may also be referenced as Interleukin-6, IL-6, and B-cell stimulatory factor 2 in the literature or in databases. When comparing results across studies, confirm that the reported analyte refers to the same molecule, species context, and molecular form (e.g., precursor vs mature protein, or soluble vs membrane-associated forms).

Why it matters in research

Understanding how IL-6 relates to innate and adaptive immune responses, cytokine signaling networks, host–pathogen interactions, and immune cell activation and trafficking in immunology, cardiovascular, and cancer research.
Interpreting shifts in IL-6 levels alongside other pathway components or complementary markers.
Connecting molecular changes to phenotypes such as inflammation, remodeling, metabolism shifts, or cell-state transitions (context-dependent).

Molecular forms and interpretation

For some targets, isoforms, proteolytic processing, or post-translational modifications (such as phosphorylation or glycosylation) can influence function and apparent abundance. If multiple molecular forms are expected in your model, align interpretation with the form most relevant to the biological question.

Disease and translational relevance

IL-6 has been investigated across diverse physiological and disease contexts, and changes in its abundance have been reported in areas aligned with immunology, cardiovascular, and cancer studies. These associations are interpreted as research findings rather than diagnostic or therapeutic claims, and they should be evaluated alongside model-specific covariates and study design.

Details

Specifications

TargetInterleukin-6

UniProt #P20607

SpeciesRat

ReactivityRat

ApplicationsELISA, Cytokine Secretion Assay

Sample type(s)Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Sensitivity37.5pg/ml

Detection range62.5-4000pg/ml

Detection methodSandwich ELISA, Double Antibody

Assay time4 hours

Storage2-8 °C for 12 months

Catalog no. (Mfr.)ER0042

Main SKUBHE10800208

Applications

Applications & how to use

ELISA

Quantifies a soluble analyte using antibody capture and enzyme-linked detection on a microplate, giving concentration readouts against a standard curve.

Cytokine Secretion Assay

Evidence

Validation & QC

Validation data for this product isn’t published yet.

Need specific QC or validation results — titer, purity, endotoxin, or assay data — for your application? Our scientific team can share what is available for this product and lot on request.

Request validation data

Manufacturing

How it's made & quality control

Supplier quality control

ELISA kits and immunoassay reagents are validated for sensitivity, specificity, recovery and linearity, with lot-specific standard curves supplied.

Kit inserts document detection range, sample types and intra-/inter-assay CVs; antibodies include validated applications.

Quality system certified to ISO 9001:2015; lot-specific Certificate of Analysis provided.

Supplier-level quality statement. For lot-specific results, request the Certificate of Analysis for your batch.

Resources

Documents & downloads

Manual PDF MSDS PDF

Questions

Frequently asked questions

?What?s the plate size in FineTest? ELISA Kits?

The ELISA plate follows the standard size of microplate: 127.64 mm x 85.60 mm x 14.22 mm(L x W x H).

?How about the shelf life and stability of FineTest? ELISA Kits?

Valid for 12 months since the production date. For the shelf life of specific batch number, please check the label printed on the kit. Before delivery, all FineTest? ELISA Kits have been subject to strict quality test.

?Which cloned antibodies for FineTest? ELISA Kits are used?

These information is proprietary. Please contact us to learn more about clonality (polyclonality or monoclonality) and host species.

?Can I mix reagents from different batches of FineTest? ELISA Kits?

Not suggested. ELISA reagents are optimized for specific batch.

?Can FineTest? ELISA Kits be used partially?

Yes. The ELISA plate is dismounted. Enough component volumes are offered by 96T ELISA kit, supporting two groups of standard curve.

?How long can the diluted lyophilized standard be stored for continual use?

Used up within 12h.

?Can standard curve be extended to any direction?

FineTest? can't support validation of standard concentration outside of standard curve. Ranges of standard curve have been validated among many batches and experimenters, showing stable and accurate performance. The lowest standard concentration is the minimized range for reliable detection results. Adding higher or lower concentration of standard may cause inconsistent signal or false positive.

?Why does detection for serum/plasma sample by FineTest? ELISA Kits require for 1/2 dilution?

Matrix components in serum/plasma can affect detection results. Blocking components in sample dilution buffer can decrease or remove the interference. The dilution can reduce the matrix difference between sample and standard to get better accuracy.

?What?s the half-life of protein in serum/plasma/cell culture supernatant?

FineTest? can't determine the half-life of protein in the sample(e.g. serum, plasma or cell culture supernatant). Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container. Avoid freeze-thaw cycle to prevent protein degradation.

?What's the expected concentration for particularly analyzing my sample?

Due to the specificity of each sample, it's hard to forecast and depend on sample preparation as well as analytical characteristics. Please contact us to get detection data for reference.

Answered by our team

Questions from researchers

No researcher questions have been answered for this product yet. Ask our technical team and we'll get back to you — approved answers are published here.

Ask our technical team

Reviewed by a scientist — typically answered within one business day.

Matches this product

$50 Off All ELISA Kits

Limited time ELISA50

10% Off Pre-Designed siRNA Sets

SIRNA10

15% Off Cancer Antibodies

Ends Sep 30 ONCO15

20% Off Transmembrane Proteins

TM20

50% Off Lab Consumables + Free Shipping

LABSAVE50

Offer

Denarase® Endonuclease — 10% Off One Order

DENARASE10

10% OFF CELL LINES-Limited-Time Offer

Ends Sep 30 CELL10

Free Sample – CellTrypase Recombinant Trypsin-Like Enzyme

Limited time offer – availa... FREESAMPLE

Free 25 kU Sample — Denarase® & Denarase® High Salt

FREESAMPLE

Free Sample – MycoFold™ Growth Factors

Limited time offer – availa... Request Free Sample

15% Off Proteins from Trusted Suppliers — Limited Time

Limited Time PT15OFF

Browse all current deals

References

Citations & references

Broad‐Spectrum ROS/RNS Scavenging Catalase‐Loaded Microreactors for Effective Oral Treatment of Inflammatory Bowel Diseases

IF: 13 Journal: Small Author: Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 199 Ren'ai Road, Suzhou, Jiangsu, 215123, P. R. China. Cited Date: 2025-05-09

Ultrasound Trigger Ultrasmall Multifunction Nanobubbles for Thrombus Visual Treatment

IF: 10 Journal: Advanced Healthcare Materials Author: Department of Ultrasound, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China. Cited Date: 2025-06-13

Sclareol alleviates synovial inflammation in knee osteoarthritis by regulating sphingolipid metabolism along the gut–bone axis

IF: 8.3 Journal: Phytomedicine Author: Department of Orthopaedics and Traumatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210023, China. Cited Date: 2025-11-28

Xiaoyao San and Guipi Tang, alone or combined, relieve postpartum depression via AGE?RAGE?mediated autophagy and apoptosis

IF: 8.3 Journal: Phytomedicine Author: Department of Traditional Chinese medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia, China. Cited Date: 2025-10-11

c-FLIP Protects Cardiac Microcirculation in Sepsis-Induced Myocardial Dysfunction Via FUNDC1-Mediated Regulation of Mitochondrial Autophagy

IF: 7.2 Journal: JACC: Basic to Translational Science Author: Department of Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. Cited Date: 2025-10-11

The dual gastro-and neuroprotective effects of curcumin loaded chitosan nanoparticles against cold restraint stress in rats

IF: 6.529 Journal: Biomedicine & Pharmacotherapy Cited Date: 2022-03-11

Zinc supplementation in normal conditions increases the pro-inflammatory cytokines

IF: 6.369 Journal: DRUG INVENTION TODAY Cited Date: 2019-08-25

Exposure to four typical heavy metals induced telomere shortening of peripheral blood mononuclear cells in relevant with declined urinary aMT6s in rats

IF: 6.2 Journal: Ecotoxicology and Environmental Safety Author: The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Department of Toxicology, Guizhou Medical University,?Guian New Area, Guizhou 561113,?China. Cited Date: 2024-08-02

The prenatal challenge with lipopolysaccharide and polyinosinic:polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of male rat offspring: a link to schizophrenia-like behaviours

IF:

Customization

Customization & sourcing

Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

Talk to a scientist

Explore

Collections featuring this product

All collections

This product is included in the curated collections below. Browse a collection to source everything for a workflow from a single cart.

Cytokine Biology 25001 products Cytokine Biology — ELISA Kits 6260 products ELISA Kits 25001 products Fine Test 21072 products IL-1 Family Signaling 4087 products Immunology ELISA Kits 6335 products

From the blog

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Get it NOW Free Sample