| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A recombinant fragment specific to human BRAF V600E mutant protein was used as the immunogen for the recombinant BRAF V600E antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Recombinant BRAF V600E Antibody is an antibody targeting BRAF. Host: Rabbit. Monoclonal. Clone: BRAF-V600E/13276R. Format: Purified. Applications: IHC-P.
Key elements and design rationale
- Target: BRAF.
- Immunogen: A recombinant fragment specific to human BRAF V600E mutant protein was used as the immunogen for the recombinant BRAF V….
- Species reactivity: Human.
- Localization: Cytoplasm.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
The BRAF gene encodes a protein that is part of the RAS-RAF-MEK-ERK signaling pathway, which regulates cell division and proliferation. The V600E mutation in the BRAF gene leads to the production of a constitutively active BRAF protein, resulting in uncontrolled cell growth and division.
Common research applications
- IHC: tissue distribution assessment; interpret with matched controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
