| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A recombinant human CDKN1B protein was used as the immunogen for the recombinant CDKN1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Recombinant CDKN1B Antibody / p27Kip1 is an antibody targeting CDKN1B. Host: Mouse. Monoclonal. Clone: rCDKN1B/13245. Format: Purified. Applications: IHC-P, WB.
Key elements and design rationale
- Target: CDKN1B.
- Immunogen: A recombinant human CDKN1B protein was used as the immunogen for the recombinant CDKN1B antibody..
- Species reactivity: Human.
- Localization: Cytoplasm, Endosome, Nucleus.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
This MAb recognizes a 27kDa protein called p27Kip1/CDKN1B/Cyclin-dependent kinase inhibitor 1B, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
- IHC: tissue distribution assessment; interpret with matched controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
