| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | 2,3-Bisphosphoglycerate Mutase Erythrocyte;2,3-Bisphosphoglycerate Synthase;2,3-Diphosphoglycerate Mutase;BPG-Dependent PGAM;BPGM;Bisphosphoglycerate Mutase;DPGM |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
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| UniProt # |
Scientific Background
Human BPGM Protein — also known as 2; 3-Bisphosphoglycerate Mutase Erythrocyte; 2; 3-Bisphosphoglycerate Synthase; 2; 3-Diphosphoglycerate Mutase; BPG-Depe — Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily. BPGM is a multifunctional enzyme. BPGM catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. It also has phosphoglycerate phosphomutase activity. BPGM plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Deficiency of BPGM increases the affinity of cells for oxygen and result in hemolytic anemia.
Research into Human BPGM Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
E. coli (Prokaryotic)C-His
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The C-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Ser2-Lys259.
Protein Specifications
| Molecular Weight (Calculated) | 31.0 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 30 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Affinity Tag | C-His |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, pH 8.0. |
| Reconstitution | Not Applicable |
| Storage Conditions | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P07738 |
| UniProt ID | P07738 |
| Expression Region | Ser2-Lys259 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH032119SpeciesHumanUniProtP07738
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Not Applicable. Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
