| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA. |
| Expression System | |
| Form | Sterile Filtered liquid formulation. |
| Formulation | |
| Product Type | |
| Protein Size | |
| Purity | |
| Source | Sf9 insect cells. |
| Species | |
| Storage | |
| Target |
Recombinant Human Centromere Protein-A is supplied as a recombinant protein for in vitro research use.
Background
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
-
Immunological functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera). - Coating concentration: 0.15-0.4 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
What is the purity of Recombinant Human Centromere Protein-A (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human Centromere Protein-A (Human) be stored?
What expression system was used to produce this protein?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
