| Field | Specification |
|---|---|
| Target | |
| Alternative names | CAPB;DRT;EK5;EPHB2;EPHT3;ERK;Ephrin type-B receptor 2;Hek5;PCBC;Tyro5 |
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| Tag | |
| Expression system | |
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| Purity | |
| Bioactivity | The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. |
| Endotoxin level | |
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| Formulation | |
| Form | Liquid |
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Scientific Background
Ephrin type-B receptor 2; also known as EphB2; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses; suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors; but does not require the kinase activity of EphB.
Product Description
Expression System: Insect (Sf9/Baculovirus)
Recombinant human EphB2 is expressed in Baculovirus-Insect Cells spanning Gly570-Val987, supplied with a N-His-GST tag. Purity is 90% as determined by reducing SDS-PAGE and the product is provided as a liquid preparation.
Protein Specifications
| Expression Host | Baculovirus-Insect Cells |
|---|---|
| Tag | N-His-GST |
| Expression Region | Gly570-Val987 |
| Calculated MW | 75.2 kDa |
| Observed MW | 65 kDa |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 3mM DTT, 10% glycerol |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Lead Time | 2-3 business days |
| Shelf Life | 12 months |
| Accession Number | P29323-3 |
| UniProt # | P29323 |
| Alternative Names | CAPB;DRT;EK5;EPHB2;EPHT3;ERK;Ephrin type-B receptor 2;Hek5;PCBC;Tyro5 |
Functional Activity
The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant proteins and matched antibodies or ELISA kits for EphB2 research from Elabscience Biotechnology Co Ltd, or contact us for alternative tags and expression formats.
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