| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | FA;FA-H;FA1;FAA;FACA;FAH;FANCH |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human FANCA/FACA Protein — also known as FA; FA-H; FA1; FAA; FACA; FAH; FANCH — FANCA is one of the six known Fanconi anemia gene products (FANCA, FANCC, FANCD2, FANCE, FANCF, and FANCG proteins). Fanconi anemia (FA) is a genetic disorder predisposing to aplastic anemia and cancer characterized by hypersensitivity to DNA-damaging agents and oxidative stress. FANCA associates with the IκB kinase (IKK) signalsome via interaction with IKK2. Components of the FANCA complex undergo rapid, stimulus-dependent changes in phosphorylation, which are blocked by kinase-inactive IKK2.
Research into Human FANCA/FACA Protein supports investigations in cellular signaling and fundamental cell biology, with implications for epigenetics & gene regulation.
Product Description
Baculovirus-Insect CellsN-His
Produced in Baculovirus-Insect Cells, yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The N-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Met 1-Cys 297.
Protein Specifications
| Molecular Weight (Calculated) | 35.2 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 35 kDa |
| Purity | > 85% as determined by reducing SDS-PAGE. |
| Affinity Tag | N-His |
| Expression System | Baculovirus-Insect Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile 20mM Tris, 200mM NaCl, pH 8.5, 20% glycerol, 0.1% Triton, 1mM TCEP Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Plea |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | AAH08979.1 |
| UniProt ID | O15360 |
| Expression Region | Met 1-Cys 297 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH030946SpeciesHumanUniProtO15360
This protein is produced in baculovirus-infected insect cells. Baculovirus/insect expression provides intermediate post-translational modifications between bacterial and mammalian systems.
Purity: > 85% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
