| Field | Specification |
|---|---|
| Accession Number | |
| Alternative Names | CD33;Myeloid Cell Surface Antigen CD33;SIGLEC3;Sialic Acid-Binding Ig-Like Lectin 3;Siglec-3;gp67 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human SIGLEC3/CD33 Protein — also known as CD33; Myeloid Cell Surface Antigen CD33; SIGLEC3; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67 — CD33 is a type I Lectin belonging to the Ig superfamily. CD33 contains an N terminal Ig like V type domain, which mediates sialic acid binding, followed by one Ig like C2 type domain, a transmembrane region and a cytoplasmic tail containing two conserved immunoreceptor tyrosine based inhibition motifs (ITIMs). Eleven human Siglecs have been characterized. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. CD33 related Siglecs have differential expression p
Research into Human SIGLEC3/CD33 Protein supports investigations in immune regulation and inflammatory responses, with implications for cytokine biology.
Product Description
HEK293 Mammalian CellsC-Fc-His
Produced in HEK293 Mammalian Cells, preserving native glycosylation patterns and supporting biological activity in cell-based assays.
The C-Fc-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Asp18-His259.
Protein Specifications
| Molecular Weight (Calculated) | 55.0 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 80 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Affinity Tag | C-Fc-His |
| Expression System | HEK293 Mammalian Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM EDTA, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please ref |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | AAH28152.1 |
| UniProt ID | P20138 |
| Expression Region | Asp18-His259 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH033054SpeciesHumanUniProtP20138
This protein is produced in HEK293 mammalian cells. Mammalian expression preserves glycosylation and folding closest to the native protein, supporting biological activity in cell assays.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
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