| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A, Ubiquitin G76A. |
| Expression System | |
| Form | Sterile Filtered clear solution. |
| Formulation | |
| Product Type | |
| Purity | |
| Source | Escherichia Coli. |
| Species | |
| Storage | |
| Target |
Recombinant Human Ubiquitin Gly76 to Ala76 mutation is supplied as a recombinant protein for in vitro research use.
Background
The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins. Ubiquitination occurs through sequential steps catalyzed by activating (E1), conjugating (E2), and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ub’s C-terminal glycine residue (G76) and a lysine residue of the target protein, although N-terminal ubiquitination is also known. Outcomes of this modification include destabilization of the conjugated protein, altered protein trafficking and functional modulation. After targeting the protein for specific localizations, ubiquitin is released from the substrate by deubiquitinating enzymes. A mutant ubiquitin, having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process. As consequence, the Ub derivative becomes irreversibly conjugated to protein, shifting the equilibrium between the bound and unbound form in the direction of conjugation, at the expense of the free form.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
What is the purity of Recombinant Human Ubiquitin Gly76 to Ala76 mutation (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human Ubiquitin Gly76 to Ala76 mutation (Human) be stored?
What expression system was used to produce this protein?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
