| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik. |
| Biological Activity | |
| Cellular Localization | |
| Expression System | |
| Form | Filtered White lyophilized (freeze-dried) powder. |
| Formulation | |
| Product Type | |
| Protein Length | |
| Protein Size | |
| Purity | |
| Solubility | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility. |
| Source | Escherichia Coli. |
| Species | |
| Storage | |
| Target |
Recombinant Human Visfatin is supplied as a recombinant protein for in vitro research use.
Background
Excess adiposity is the most important risk in the development of type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor-a, and IL-6, that modulate sensitivity and appear to play an important role in the pathogenesis, diabetes, dyslipidemia, inflammation, and atherosclerosis. Visfatin, also known as pre-B cell colony-enhancing factor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity. Visfatin exerts mimetic effects that are dose-dependent and quantitatively similar to stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its mimetic effects, visfatin was as effective in reducing hyperglycemia in deficient diabetic mice. Visfatin was also found to be bound to and activate receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin did not compete for binding to the receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.
About Visfatin Human Visfatin is a cytokine expressed in visceral fat that was originally isolated as a secreted element that synergized with stem cell factors and IL-7. One of its main functions is to enhance the development of B cell precursors. The cytokine is also known as the “Pre-B Cell Colony-Enhancing Factor (PBEF).” It has been identified in vertebrates, including mice and humans, and it’s being studied due to its link to inflammatory conditions, beta cell function, and cardiovascular disease. What’s the Function of Visfatin Human Recombinant? Visfatin human recombinant is produced in E. Coli. It’s a single, non-glycosylated, polypeptide chain that contains 466 amino acids, it’s purified by FLAG-affinity chromatography, and it contains a total molecular mass of 52.6 kDa. What Are the Main Applications of Visfatin Human Recombinant? The cytokine is being researched because of its involvement in glucose homeostasis, dysregulation in biosynthesis and signal transduction, and the pathogenesis of diabetes. Visfatin human recombinant is tailored exclusively for laboratory research, ensuring experts can get further answers regarding the cytokine’s involvement in different processes, including pathogenesis, diabetes, inflammation, dyslipidemia, and atherosclerosis. Findings can also help during the identification of high-risk people for cardiovascular disease and diabetes.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
What is the purity of Recombinant Human Visfatin (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human Visfatin (Human) be stored?
What expression system was used to produce this protein?
Is this protein biologically active?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
