| Field | Specification |
|---|---|
| Target | |
| Alternative names | 4833442P21Rik Protein;CD16-2 Protein;FcgRIV Protein;FcgammaRIV Protein;Fcgr3a Protein;Fcrl3 Protein;Mouse |
| UniProt # | |
| Accession number | |
| Species | |
| Applications | |
| Conjugate | |
| Tag | |
| Expression system | |
| Expression region | |
| Molecular weight | |
| Purity | |
| Bioactivity | 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA. 2. Labeling ratio of biotin to protein: 0.6 |
| Endotoxin level | |
| Formulation | |
| Form | Lyophilized |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc γ receptors (FcR), and they are Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc γ RIII (CD16) group.
Product Description
Expression System: HEK293
Recombinant mouse CD16-2/FCGR4 is expressed in HEK293 Cells spanning Met 1-Gln 203, supplied with a C-His-Avi tag. Purity is 95% as determined by reducing SDS-PAGE and the product is provided as a lyophilized preparation.
Protein Specifications
| Expression Host | HEK293 Cells |
|---|---|
| Tag | C-His-Avi |
| Expression Region | Met 1-Gln 203 |
| Calculated MW | 24.2 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Lead Time | 2-3 business days |
| Shelf Life | 12 months |
| Accession Number | NP_653142.2 |
| UniProt # | A0A0B4J1G0 |
| Alternative Names | 4833442P21Rik Protein;CD16-2 Protein;FcgRIV Protein;FcgammaRIV Protein;Fcgr3a Protein;Fcrl3 Protein;Mouse |
Functional Activity
1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA. 2. Labeling ratio of biotin to protein: 0.6
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant proteins and matched antibodies or ELISA kits for CD16-2/FCGR4 research from Elabscience Biotechnology Co Ltd, or contact us for alternative tags and expression formats.
Can't Find What You're Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
