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Overview
Red Carboxyl Fluorescent Nanoparticles are europium chelated carboxylate fluorescent microspheres that are monodispersed and have a broad Stokes Shift and a very long lifetime (milliseconds). The extended lifetime enables the europium chelate beads to be used in a wide range of time resolved fluorescence applications including lateral flow assays, and immune/histological research.
Use of the europium beads lowers by several fold the detection limit of an assay from that achieved with a conventional fluorophore, as the europium beads have higher fluorescence intensity and eliminate background interference from relatively short-lived matrix fluorescence.
Key Features
- Carboxylate microspheres
- Low non-specific binding
- High colloidal stability
- High photostability
- Narrow size distribution
Applications
- Quantitative lateral flow assays
- Blood flow determinationr
- Tracing
- Flow cytometry
- In vivo imaging and calibration of imaging
Physical & Chemical Properties
- Excitation Wavelength: 365 nm
- Chemical Stability: The product is chemically stable under standard ambient conditions (room temperature).
- Incompatible Materials: Strong oxidizing agents
- Conditions to Avoid: Exposure to moisture.
Red Carboxyl Fluorescent Nanoparticles are monodisperse polystyrene microspheres (100–800 nm) with carboxylate surface groups and red fluorescent dye embedded in the polymer matrix (emission ~615 nm). Their distinct emission wavelength enables multiplexing with green-fluorescent nanoparticles in the same assay.
Applications include lateral flow assay (LFA) development, blood flow determination, cell tracing and tracking, flow cytometry calibration, in vivo fluorescence imaging calibration, and multiplexed bead-based assay platforms combined with green or other fluorescent nanoparticles.
The green (525 nm) and red (615 nm) emission channels can be separated by standard bandpass optical filters. Using both colors simultaneously allows two-analyte detection in the same lateral flow strip or imaging experiment with minimal spectral crosstalk.
Available in 100, 200, 300, 400, 500, 600, and 800 nm, plus a combo pack. Smaller sizes flow more uniformly along nitrocellulose membranes in LFA and have lower steric hindrance. Larger sizes provide stronger per-particle signal. Size optimization is typically required for each specific LFA test line.
Conjugation uses standard EDC/NHS chemistry to couple amine-bearing antibodies or proteins to the carboxylate surface groups. The polymer matrix confines the fluorescent dye, preventing leaching during conjugation or assay conditions.
The following customization and add-on services are available for this product through the supplier. For inquiries and pricing, contact support@biohippo.com.
Customization Options
- Custom Particle Sizes: Fluorescent nanoparticle sizes beyond the standard catalog range can be manufactured with narrow size distribution as needed for specific lateral flow or imaging applications.
- Custom Surface Functionalization: Particles with alternative surface groups (amine, streptavidin, or custom coatings) can be produced for targeted bioconjugation strategies.
- Custom Conjugation Service: Pre-conjugated fluorescent nanoparticle–antibody conjugates can be prepared using your supplied antibody.
- Lateral Flow Assay Development: Full lateral flow immunoassay (LFA) development services using latex fluorescent beads — including large colloidal gold-based and fluorescent bead-based LFA — are available.
- Bulk & OEM Manufacturing: OEM latex fluorescent bead manufacturing for lateral flow immunoassay is available at production scale.
To inquire about customization options, request a quote, or discuss OEM manufacturing, contact support@biohippo.com.
- Yang Z et al. (2026). Europium nanoparticle label/lateral flow test strip integrated with a 3D-printed fluorescence smartphone reader for detection of melatonin in human blood. Nanoscale Horiz. DOI: 10.1039/d5nh00853k PMID: 41810487
- Zheng H et al. (2026). Development of a dual-nanobody sandwich immunochromatographic strip based on a site-directed conjugation strategy for ultrasensitive detection of staphylococcal enterotoxin B (SEB). Anal Chim Acta. DOI: 10.1016/j.aca.2026.345417 PMID: 41965301
- Sheng L et al. (2026). Development of a DMSNs@CuInS/ZnS-based flow immunochromatographic strip method for gliadin detection. Talanta. DOI: 10.1016/j.talanta.2026.129354 PMID: 41512624
- Lu H et al. (2026). Lanthanide-doped luminescent lateral flow immunoassay for the ultrasensitive and quick detection of methyltestosterone in fish samples. Anal Methods. DOI: 10.1039/d6ay00063k PMID: 41769960
