| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RNF220 recombinant protein (Position: M1-L566) was used as the immunogen for the RNF220 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RNF220 Antibody / Ring finger protein 220 is a anti-RNF220 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, nucleus.
Key elements and design rationale
- Target: RNF220
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
At the cellular level, Ring finger protein 220 localizes to the nucleus and nuclear lamina, where it participates in multiprotein complexes that coordinate transcriptional repression and activation. Depending on context, Ring finger protein 220 may promote degradation of certain repressors or stabilize selected activators, thereby steering lineage programs and adaptive responses. In addition, Ring finger protein 220 has been connected to canonical Wnt readouts in some systems, consistent with its broader role in influencing transcription factor availability. Because E3 ligases are nodal points in proteostasis and signaling, their measurement can reveal how protein quality control intersects with cell identity and disease.
Researchers apply RNF220 Antibody in immunoblotting to quantify endogenous protein and track changes after perturbing proteasome activity or E2 partner availability. In immunofluorescence, the antibody highlights nuclear and lamina-associated patterns that can be correlated with chromatin markers or transcriptional co-regulators. Co-immunoprecipitation with RNF220 Antibody enables the capture of complexes for proteomic or ubiquitin-site analysis, helping define substrate spectra under differentiating or stress conditions. In translational studies, measuring Ring finger protein 220 may clarify mechanisms underlying white matter disorders and other developmental phenotypes where ubiquitin signaling is disrupted.
The RNF220 Antibody from
As the field expands its view of how E3 ligases sculpt transcriptional landscapes, RNF220 Antibody offers a reliable handle on an underexplored regulator. Its versatility across imaging, pulldown, and quantitative immunoblotting makes it a cornerstone reagent for decoding how ubiquitin-dependent control of transcription factors and co-regulators shapes lineage choice, adaptation, and disease progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
