| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RNF25 recombinant protein (Position: E44-R344) was used as the immunogen for the RNF25 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RNF25 Antibody / Ring finger protein 25 is a anti-RNF25 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: RNF25
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, WB
Biological background
Functionally, RNF25 antibody identifies a 459-amino-acid cytoplasmic and nuclear protein containing a C3H2C3-type RING finger domain essential for ubiquitin ligase activity. RNF25 acts as a regulatory adapter that promotes the assembly of ubiquitin ligase complexes and modulates the stability of key signaling molecules. It interacts with components of the NF-kappaB signaling pathway, such as TRAF2 and RelA/p65, thereby influencing inflammatory gene expression and apoptosis.
The RNF25 gene is located on chromosome 15q22.31 and encodes a protein expressed in multiple tissues, including brain, liver, and immune cells. It functions in the ubiquitin-proteasome system to regulate protein turnover and signaling dynamics. RNF25 has been shown to enhance NF-kappaB transcriptional activity by stabilizing the p65 subunit, amplifying responses to cytokines and stress stimuli. It may also participate in synaptic signaling and neuronal plasticity through regulation of protein degradation in neurons.
Beyond its role in NF-kappaB signaling, RNF25 influences other cellular processes such as apoptosis, transcriptional repression, and immune modulation. It interacts with Smurf1 and other E3 ligases to fine-tune ubiquitin-dependent degradation networks. Dysregulation of RNF25 expression or activity has been linked to cancer progression, chronic inflammation, and neurodegenerative disease. Overexpression of RNF25 promotes survival pathways, while loss of function increases sensitivity to apoptotic stimuli.
RNF25 antibody is widely used in research focused on protein degradation, signal transduction, and transcriptional regulation. It is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect RNF25 expression and localization. This antibody supports studies exploring the ubiquitin-proteasome system, NF-kappaB activation, and E3 ligase interactions. In disease models, RNF25 serves as a biomarker of altered ubiquitin signaling and inflammatory response.
Structurally, RNF25 features a RING-type zinc-binding domain, a coiled-coil region for protein interactions, and nuclear localization motifs that enable shuttling between cytoplasm and nucleus. Post-translational regulation includes phosphorylation and auto-ubiquitination that modulate its ligase activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
