| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human RNF7 was used as the immunogen for the RNF7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RNF7 Antibody / Ring finger protein 7 is a anti-RNF7 Rabbit antibody Recombinant Rabbit Monoclonal clone 30R91 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RNF7
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30R91, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Ring finger protein 7 has multiple cellular roles, including redox regulation, neddylation, and tumor suppressor control. By facilitating ubiquitination, RNF7 ensures timely degradation of proteins such as p27 and IkappaB, which govern cell cycle checkpoints and NF kappa B signaling respectively. Studies using RNF7 antibody have shown that disruption of this E3 ligase leads to aberrant accumulation of these regulators, causing uncontrolled proliferation or impaired immune responses. RNF7 also interacts with antioxidant pathways by mediating degradation of pro oxidant proteins, supporting redox balance in the cell.
Altered expression of Ring finger protein 7 has been implicated in cancer and degenerative diseases. Overexpression of RNF7 has been reported in several human cancers, including prostate, pancreatic, and lung carcinoma, where it may promote tumorigenesis by reducing levels of pro apoptotic proteins. Conversely, loss of function mutations impair ubiquitin ligase activity, leading to genomic instability. RNF7 antibody has been applied to studies that link this factor to chemotherapy resistance and cancer progression, making it an attractive target for drug discovery.
RNF7 antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting detects expression in cancer cell lines, while immunohistochemistry demonstrates its nuclear and cytoplasmic distribution in tissue sections. Immunoprecipitation with RNF7 antibody enables isolation of the SCF complex for functional studies. Together, these applications allow researchers to investigate its role in proteostasis, stress response, and tumor biology.
By providing validated RNF7 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
