| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 63-90 from the human protein was used as the immunogen for this SCN1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCN1B Antibody / Sodium channel subunit beta-1 is an antibody targeting SCN1B, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: SCN1B.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Human, Mouse.
- Listed applications: WB, IHC-P, FACS (refer to on-page specifications for application-specific guidance).
Biological background
Sodium channel subunit beta-1 (SCN1B) is a protein that is essential for the proper functioning of sodium channels in our cells. Sodium channels are responsible for the movement of sodium ions across cell membranes, playing a vital role in regulating cell excitability and communication. SCN1B is one of several subunits that work together to form functional sodium channels, with each subunit contributing to the overall activity and properties of the channel. One of the key roles of SCN1B is to modulate the activity of sodium channels, influencing their opening and closing dynamics. This modulation is crucial for controlling the flow of sodium ions into cells, which is essential for proper nerve signaling and muscle function. In addition to its role in regulating sodium channel activity, SCN1B also helps to stabilize the structure of sodium channels and promote their trafficking to the cell membrane. Research has shown that mutations in the SCN1B gene can lead to disruptions in sodium channel function, resulting in a variety of neurological and cardiovascular disorders. For example, mutations in SCN1B have been linked to epilepsy, as well as arrhythmias and heart failure.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Flow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
