| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human SEC62 was used as the immunogen for the SEC62 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SEC62 Antibody / Translocation protein SEC62 is a anti-SEC62 Rabbit antibody Recombinant Rabbit Monoclonal clone 31S64 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SEC62
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31S64, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Translocation protein SEC62 cooperates with the SEC61 channel and SEC63 cochaperone to mediate insertion of polypeptides into the endoplasmic reticulum lumen. Research using SEC62 antibody has shown that it functions particularly in the import of short signal peptide proteins and stress-inducible proteins. This activity helps maintain proteostasis under conditions of cellular stress, ensuring that unfolded or newly synthesized proteins are efficiently imported and processed.
Studies with SEC62 antibody have revealed that the protein also contributes to the unfolded protein response and endoplasmic reticulum stress adaptation. By regulating the influx of client proteins, Translocation protein SEC62 limits accumulation of misfolded proteins and protects cells from apoptosis. These findings underscore its protective role in maintaining endoplasmic reticulum homeostasis.
In cancer biology, overexpression of SEC62 has been identified in lung, prostate, and thyroid cancers. Research using SEC62 antibody has demonstrated that elevated expression enhances stress tolerance, migration, and invasion, linking the protein to aggressive tumor phenotypes. Because of these properties, SEC62 is being explored as a prognostic biomarker and therapeutic target in oncology.
SEC62 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects SEC62 expression under stress conditions, immunohistochemistry highlights localization to the endoplasmic reticulum in tissues, and immunofluorescence shows its membrane association with endoplasmic reticulum structures. These applications make SEC62 antibody an important reagent in cell biology and disease research.
By supplying validated SEC62 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
