| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SETX recombinant protein (Position: E29-R2544) was used as the immunogen for the Senataxin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Senataxin Antibody / SETX is a anti-SETX Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SETX
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, Senataxin is a 2677-amino-acid helicase belonging to the superfamily 1 (SF1) of DNA/RNA helicases. It contains a C-terminal helicase domain with conserved Walker A and B motifs for ATP binding and hydrolysis, an N-terminal region that mediates protein-protein interactions, and nuclear localization sequences. The helicase activity of Senataxin resolves R-loop structures formed during transcription, preventing collisions between transcription and replication machinery. Co-localization studies show Senataxin enriched in nuclear foci associated with sites of active transcription and DNA repair.
Functionally, Senataxin maintains genome integrity by resolving R-loops and facilitating proper termination of RNA polymerase II transcription. It contributes to DNA double-strand break repair via homologous recombination and regulates transcription-coupled nucleotide excision repair. Senataxin also modulates RNA maturation by interacting with RNA processing factors such as XRN2 and the exosome complex. In neurons, it supports axonal maintenance and stress granule dynamics, while in germ cells, it regulates meiotic recombination and spermatogenesis.
Mutations in the SETX gene are linked to several neurodegenerative and neuromuscular disorders, including ataxia with oculomotor apraxia type 2 (AOA2) and juvenile amyotrophic lateral sclerosis type 4 (ALS4). These conditions are characterized by progressive neurodegeneration and impaired DNA repair capacity. Senataxin deficiency leads to accumulation of R-loops, transcriptional stress, and genomic instability. Pathway associations include RNA processing, DNA damage response, and transcription regulation. During development, Senataxin expression supports neuronal differentiation and genome maintenance in post-mitotic cells.
The Senataxin antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
