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SK-LU-1 is a human lung adenocarcinoma cell line widely used in cancer research, particularly in studies focused on non-small cell lung cancer (NSCLC). As a cisplatin-sensitive cell line, SK-LU-1 is often employed in studies evaluating chemotherapy resistance, cancer cell cycle progression, and apoptosis mechanisms. One of the defining features of SK-LU-1 is its utility in assessing the cytotoxic effects of various anti-cancer compounds, including those that modulate the cell cycle or induce apoptosis through targeted therapies. For instance, certain 6-substituted imidazopyridine derivatives have been shown to induce G2/M phase arrest and apoptosis in SK-LU-1 cells, indicating that these compounds may inhibit cyclin-dependent kinases (CDKs) involved in cancer cell division.
Additionally, SK-LU-1 cells have been used in studies exploring the immunomodulatory effects of agents like melatonin. In co-culture experiments with peripheral blood mononuclear cells (PBMCs), melatonin was shown to enhance the immune system's ability to induce apoptosis in SK-LU-1 cells. The treatment led to increased oxidative stress, reduced glutathione (GSH) levels, and cell cycle arrest at the G0/G1 phase, suggesting that melatonin may have potential as a supplementary treatment in NSCLC by boosting immune response and promoting cancer cell death.
Overall, SK-LU-1 provides a robust in vitro model for studying lung adenocarcinoma and testing novel therapeutic agents, including those that target the cell cycle, induce apoptosis, or modulate immune responses. Its responsiveness to chemotherapeutic agents like cisplatin and the wide range of experimental data available make it an important tool in NSCLC research.
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- Protein expression: p53 positive
- Antigen expression: Blood Type O, Rh+, HLA Aw24, Aw32, B27, Bw41
- Isoenzymes: Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 2, AK-1, 1, GLO-1, 2, G6PD, B
- Tumorigenic: Yes, in immunotolerant rats and nu-nu mice
- Karyotype: The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q,11q), 11q+, t(13,?), 16q+, t(12q, 18q). M10, t(2q,13q), i(15), and ?t(xp,21q) occurred in all S metaphases, and t(1p,?), t(1p,14q), t(16,?), and t(14,21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, x chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation. Phenotype Frequency Product: 0.00003
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: 2 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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