| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Alpha Synuclein/SNCA recombinant protein (Position: M1-D135) was used as the immunogen for the SNCA antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNCA Antibody / Alpha Synuclein is a anti-SNCA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: SNCA
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Alpha-synuclein belongs to the synuclein family, which also includes Beta- and Gamma-synuclein. Structurally, it consists of three domains: an amphipathic N-terminal region that binds lipid membranes, a hydrophobic central non-amyloid-beta component (NAC) domain that promotes aggregation, and a highly acidic C-terminal region that interacts with synaptic vesicle components. These domains allow Alpha-synuclein to reversibly associate with synaptic vesicle membranes and regulate vesicle clustering, docking, and fusion events required for neurotransmitter release.
The SNCA antibody is widely used in neuroscience, neurodegenerative disease, and cellular biology research to study protein aggregation, synaptic function, and neuronal homeostasis. Western blot analysis identifies a 17 kilodalton band corresponding to Alpha-synuclein, while immunohistochemistry and immunofluorescence reveal diffuse cytoplasmic and presynaptic punctate staining in neurons. In pathological tissue, the antibody recognizes characteristic Lewy bodies and Lewy neurites formed by Alpha-synuclein aggregates, providing a valuable diagnostic marker for Parkinson's disease and dementia with Lewy bodies.
Physiologically, Alpha-synuclein interacts with SNARE complex proteins such as VAMP2 and synaptobrevin to facilitate vesicle fusion, while regulating dopamine release and synaptic plasticity. However, environmental stress, oxidative damage, or genetic mutations (e.g., A53T, A30P, E46K) can cause Alpha-synuclein misfolding and fibril formation. These aggregates trigger mitochondrial dysfunction, ER stress, and neuronal death through toxic oligomer formation. The SNCA antibody is an essential tool for studying these mechanisms and evaluating therapeutic interventions aimed at reducing aggregation or promoting clearance via autophagy and proteasomal degradation.
Alpha-synuclein also plays a role in non-neuronal systems, including erythrocytes and the enteric nervous system, where it may influence vesicular trafficking and lipid metabolism. Dysregulation of SNCA transcription and post-translational modifications such as phosphorylation (Ser129), nitration, or ubiquitination modulate its aggregation behavior and toxicity. The SNCA antibody supports high-resolution mapping of these biochemical changes, helping researchers understand disease progression and identify novel treatment strategies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
