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The T98G cell line is a human glioblastoma multiforme model derived from a 61-year-old male patient. It was established to study the molecular mechanisms of tumorigenesis, cellular proliferation, and transformation. T98G cells exhibit a unique combination of both normal and transformed cellular characteristics, which makes them a valuable model for investigating cancer biology. Specifically, while T98G cells are immortal and capable of anchorage-independent growth, they retain the ability to undergo G1 phase arrest under stationary-phase conditions, a property typically associated with normal cells.
In terms of growth characteristics, T98G cells exhibit anchorage independence, as demonstrated by their ability to form colonies in methylcellulose, a semi-solid medium. However, unlike many transformed cell lines, they arrest in the G1 phase of the cell cycle when subjected to conditions of high cell density or low serum concentration. This unique ability to undergo G1 arrest under these conditions sets T98G apart from other cancer cell lines, such as HeLa or the parental T98 cells, which continue to proliferate under similar circumstances. This phenotype suggests that while T98G cells are transformed, they retain certain regulatory mechanisms that control cell cycle progression.
Cytogenetically, T98G cells are highly aneuploid, with a modal chromosome number of 124-126, indicating significant chromosomal instability. The presence of marker chromosomes and minute chromosomes in their karyotype further reflects the genetic alterations commonly associated with glioblastoma multiforme. Despite their transformed and aneuploid nature, T98G cells are non-tumorigenic when injected into nude mice, demonstrating that anchorage independence alone is insufficient for tumorigenicity.
The T98G cell line serves as an important tool for studying glioblastoma progression, cell cycle regulation, and the interplay between normal and transformed cellular behaviors. Its ability to retain aspects of normal G1 arrest makes it a particularly useful model for exploring mechanisms underlying cellular transformation, cell cycle checkpoints, and therapeutic targets for glioblastoma.
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- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- doublingTime: 40 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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