| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TGF Beta 1/TGFB1 recombinant protein (Position: A279-H347) was used as the immunogen for the TGFB1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TGFB1 Antibody / Transforming growth factor beta-1 is a anti-TGFB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TGFB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Transforming growth factor beta-1 binds to type I and type II TGF-beta receptors, initiating phosphorylation of SMAD transcription factors that regulate gene expression. This signaling pathway influences processes such as epithelial-to-mesenchymal transition (EMT), fibrosis, angiogenesis, and immune tolerance. The TGFB1 antibody enables quantification of protein levels and distribution in tissues, helping elucidate how this cytokine coordinates cellular communication during development and wound healing.
Dysregulated TGF-beta1 signaling contributes to numerous diseases, including cancer, pulmonary fibrosis, liver cirrhosis, and autoimmune disorders. Overproduction drives fibrotic remodeling through excessive extracellular matrix deposition, while loss of function can impair immune suppression and tissue homeostasis. The TGFB1 antibody is a critical tool for analyzing these disease mechanisms, allowing assessment of active and latent forms of the cytokine. It is widely used in immunohistochemical detection of TGF-beta1 expression in fibrotic lesions, tumors, and inflamed tissues.
In cancer, Transforming growth factor beta-1 exhibits dual roles�acting as a tumor suppressor in early stages and a tumor promoter in advanced disease. It enhances epithelial cell migration and invasion by inducing EMT and modifying the tumor microenvironment. The TGFB1 antibody allows researchers to explore these context-dependent effects, providing insight into how TGF-beta1 transitions from growth inhibitory to pro-metastatic functions. In immunology, this cytokine maintains peripheral tolerance by inducing regulatory T cells and suppressing inflammatory responses.
The TGFB1 antibody performs effectively in western blotting, ELISA, and immunohistochemistry. It provides robust detection of both precursor and mature forms of Transforming growth factor beta-1.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
