| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse TIGAR recombinant protein (Position: M1-H269) was used as the immunogen for the TIGAR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator is a anti-TIGAR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TIGAR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
TIGAR is encoded by the TIGAR gene located on human chromosome 12p13.31. The protein is approximately 270 amino acids in length and structurally resembles the bisphosphatase domain of phosphofructokinase-2/fructose-2,6-bisphosphatase (PFKFB) enzymes. However, TIGAR lacks kinase activity and instead functions primarily as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate and thereby suppressing glycolysis while promoting the pentose phosphate pathway (PPP).
The TIGAR antibody detects a 30 kilodalton band by western blot and shows cytoplasmic and nuclear localization under immunofluorescence. Through its enzymatic activity, TIGAR decreases glycolytic intermediates and redirects glucose metabolism toward the PPP, enhancing production of NADPH and glutathione for antioxidant defense. This metabolic shift protects cells against oxidative stress and apoptosis, particularly under conditions of DNA damage or metabolic stress.
Regulated directly by p53, TIGAR expression serves as a metabolic checkpoint balancing energy generation and redox homeostasis. In cancer, overexpression of TIGAR supports tumor cell survival by reducing reactive oxygen species (ROS) and maintaining mitochondrial integrity. Conversely, TIGAR inhibition sensitizes cells to oxidative damage and enhances the efficacy of chemotherapeutic agents.
Beyond cancer metabolism, TIGAR plays roles in neuroprotection, cardiac remodeling, and ischemic tolerance. In neurons, TIGAR preserves mitochondrial function and reduces cell death after hypoxic injury. In cardiac tissue, TIGAR activation supports energy recovery following ischemia. These observations establish TIGAR as a critical regulator of metabolic plasticity and stress resilience.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
