| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human TNFAIP8 was used as the immunogen for the TNFAIP8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TNFAIP8 Antibody / Tumor necrosis factor alpha induced protein 8 is a anti-TNFAIP8 Rabbit antibody Recombinant Rabbit Monoclonal clone 30T22 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: TNFAIP8
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30T22, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
TNFAIP8 is induced by TNF signaling and is involved in modulating NF kappa B activity. Studies indicate that it can inhibit caspase activation and reduce apoptosis, thereby promoting cell survival under stress conditions. By altering apoptosis thresholds, TNFAIP8 helps cancer cells persist in hostile microenvironments where other cells would undergo death. Detection with TNFAIP8 antibody has shown elevated expression in multiple cancers, including lung, colon, and ovarian carcinoma, where it contributes to chemoresistance and poor prognosis. Its regulation of immune signaling pathways makes it a target of interest in both immunology and oncology, as it may serve as a biomarker for aggressiveness and therapeutic resistance.
Beyond its role in apoptosis, TNFAIP8 may participate in vesicle trafficking and autophagy. It has been implicated in lipid binding and membrane dynamics, linking it to cellular stress responses and nutrient sensing. Evidence suggests that TNFAIP8 can modulate PI3K AKT signaling, further highlighting its role in integrating survival and metabolic pathways. Researchers employing TNFAIP8 antibody can explore these broader functions and their relevance in pathological conditions, including inflammatory diseases where dysregulated cell survival exacerbates tissue injury.
TNFAIP8 antibody is commonly used in western blotting, immunohistochemistry, and ELISA. Western blotting identifies isoform expression and quantitative changes, while immunohistochemistry reveals expression patterns in tumor samples and immune tissues, offering insights into microenvironmental interactions. ELISA based assays extend applications to serum measurements in clinical samples, where circulating TNFAIP8 may serve as a biomarker for disease severity. Flow cytometry applications have also been developed, enabling detection in heterogeneous cell populations.
Research into tumor biology, inflammation, and therapeutic resistance benefits from reliable antibodies such as TNFAIP8 antibody. By providing validated reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
