| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DCR2/TNFRSF10D recombinant protein (Position: T173-A379) was used as the immunogen for the TNFRSF10D antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TNFRSF10D Antibody / TRAIL-R4 / Tumor necrosis factor receptor superfamily member 10D is a anti-TNFRSF10D Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Plasma membrane, Actin filaments.
Key elements and design rationale
- Target: TNFRSF10D
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
TNFRSF10D is encoded by the TNFRSF10D gene on chromosome 8p21 and is expressed in a wide range of tissues, including immune system cells, epithelial tissues, and endothelial cells. Its ability to act as a natural antagonist to apoptosis induction has made it a focus of cancer research. In tumors, TNFRSF10D expression may influence sensitivity to TRAIL-based therapies by preventing cell death, thereby contributing to resistance. The TNFRSF10D antibody provides a means to examine receptor expression across tumor models and to assess its regulatory role in survival pathways. Beyond oncology, the receptor may also play roles in immune homeostasis and tissue protection under stress conditions.
The TNFRSF10D antibody can be applied to a variety of experimental platforms. Western blotting provides insights into receptor expression and post-translational modifications. Immunohistochemistry enables the detection of TNFRSF10D across different tissue sections, offering information about its spatial distribution. Flow cytometry can quantify receptor levels on the surface of immune cell subsets, clarifying its function in regulating TRAIL-mediated apoptosis. These experimental approaches make the TNFRSF10D antibody a versatile research tool for investigating receptor biology.
Interest in TRAIL receptor biology continues to expand as researchers explore therapeutic strategies to induce apoptosis in cancer cells. Since TNFRSF10D counteracts the apoptotic effect of TRAIL, it has been proposed as a therapeutic target for sensitizing resistant cells. The availability of the TNFRSF10D antibody through
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
