| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TNFRSF19 recombinant protein (Position: E68-L423) was used as the immunogen for the TNFRSF19 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TNFRSF19 Antibody / Tumor necrosis factor receptor superfamily member 19 is a anti-TNFRSF19 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TNFRSF19
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, TNFRSF19 antibody identifies a 417-amino-acid receptor that participates in multiple signaling pathways including NF-kappaB, JNK, and MAPK cascades. TNFRSF19 binds members of the TNF ligand family and modulates downstream transcriptional programs controlling apoptosis, cell fate determination, and inflammation. In the nervous system, TNFRSF19 plays a crucial role in axon guidance, myelination, and neuronal survival. It is expressed in developing neural tissues, keratinocytes, and various epithelial cells.
The TNFRSF19 gene is located on chromosome 13q12.11 and encodes a single-pass type I membrane protein with an extracellular cysteine-rich domain, a transmembrane segment, and an intracellular death domain-like region. This structure allows TNFRSF19 to act as both a receptor and signaling adaptor. It interacts with TRAF2 and TRADD adaptor proteins to regulate NF-kappaB activation and cell survival. In developmental processes, TNFRSF19 coordinates Wnt and BMP signaling, influencing neural crest differentiation and epidermal homeostasis.
In cancer biology, TNFRSF19 exhibits dual roles depending on cellular context. Its overexpression has been linked to glioma and pancreatic cancer progression through activation of pro-survival pathways, while in other cancers, its expression suppresses metastasis by promoting apoptosis. TNFRSF19 also participates in immune regulation, influencing macrophage activation and inflammatory cytokine production.
TNFRSF19 antibody is widely used in studies of apoptosis, neurobiology, and immune signaling. It is suitable for immunoblotting, immunohistochemistry, and flow cytometry to detect receptor localization and expression levels. In developmental biology, TNFRSF19 serves as a marker for neural crest derivatives and epithelial differentiation. Its detection provides insight into the balance between cell survival and programmed death in both normal and disease contexts.
Structurally, TNFRSF19 shares conserved cysteine-rich motifs with other TNF receptor family members that mediate ligand binding and receptor trimerization. Its cytoplasmic domain associates with adaptor proteins to propagate intracellular signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
