| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human DcR1 / CD263 was used as the immunogen for the TRAIL R3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRAIL R3 Antibody / DcR1 is a anti-R3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31T28 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: R3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31T28, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
Decoy receptor 1 is expressed in many normal tissues, particularly in the gastrointestinal tract, lungs, and liver, where it may help protect against inappropriate TRAIL mediated apoptosis. Studies using TRAIL R3 antibody have shown that its presence prevents excessive cell death, preserving tissue integrity under inflammatory conditions. In immune regulation, DcR1 may act as a safeguard, balancing the cytotoxic effects of TRAIL expressing immune cells with the need to maintain healthy tissue survival.
In cancer biology, expression of Decoy receptor 1 has been reported in multiple tumor types. By competing for TRAIL binding, DcR1 enables malignant cells to resist TRAIL induced apoptosis, thereby promoting survival. Research with TRAIL R3 antibody has demonstrated that tumors expressing high levels of DcR1 show decreased sensitivity to TRAIL based therapies. This has made TNFRSF10C a focus of interest in understanding resistance mechanisms to immunotherapy and targeted apoptosis inducing drugs.
TRAIL R3 antibody is useful in western blotting, immunohistochemistry, and flow cytometry. Western blotting confirms expression in cell lines and tissues, while immunohistochemistry demonstrates distribution across normal and cancerous tissues. Flow cytometry with TRAIL R3 antibody allows analysis of surface expression on live cells, aiding in functional studies of TRAIL signaling. These applications provide insights into how DcR1 balances death receptor signaling in health and disease.
By providing validated TRAIL R3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
