| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide specific to TNFRSF10D protein was used as the immunogen for the TRAIL-R4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRAIL-R4 Antibody / TNFRSF10D / TRUNDD is a anti-TRAIL-R4 Rabbit antibody Recombinant Rabbit Monoclonal clone 23T76 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse. Reported localization: Plasma membrane, Actin filaments.
Key elements and design rationale
- Target: TRAIL-R4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 23T76, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P
Biological background
TRAIL R4 is expressed in a variety of tissues and cell types, including immune cells and epithelial cells. Its expression pattern suggests a role in maintaining tissue homeostasis by preventing inappropriate activation of apoptosis. In addition, TRAIL R4 has been shown to activate pro survival pathways such as NF kappa B and PI3K Akt signaling, further supporting its function as a regulator of cell fate. These properties distinguish it from TRAIL receptors DR4 and DR5, which contain functional death domains and induce apoptosis.
Research has linked TNFRSF10D to tumor biology. Overexpression of TRAIL R4 in cancer cells provides resistance to TRAIL mediated killing, allowing malignant cells to evade immune destruction. This mechanism contributes to tumor progression and therapeutic resistance in cancers such as colon, breast, and lung carcinoma. Because of this protective effect, TRAIL R4 is studied as both a biomarker and a potential target for overcoming resistance in TRAIL based therapies. Its role in modulating survival pathways also makes it relevant in studies of inflammation and immune regulation.
At the molecular level, TRAIL R4 contains extracellular cysteine rich domains typical of TNF receptors but a truncated intracellular domain that lacks apoptotic signaling capacity. This structure enables it to compete with death inducing TRAIL receptors for ligand binding, thereby controlling the balance between apoptosis and survival. Functional studies indicate that TRAIL R4 can redirect TRAIL signaling toward non apoptotic outcomes, influencing proliferation and inflammatory responses.
The TRAIL R4 antibody is widely used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to measure protein expression and localization. These applications are critical for cancer research, apoptosis studies, and immune regulation. For scientists exploring tumor immune evasion, cytokine receptor signaling, or therapeutic interventions, the TRAIL R4 antibody provides a reliable detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
