| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRPV4 recombinant protein (Position: R64-D408) was used as the immunogen for the TRPV4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRPV4 Antibody / Transient receptor potential cation channel subfamily V member 4 is a anti-TRPV4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TRPV4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
TRPV4 forms tetrameric channel complexes at the plasma membrane, where calcium influx through the pore initiates downstream signaling cascades. It interacts with cytoskeletal and scaffolding proteins including PACSIN3, AKAP150, and actin-binding proteins to fine-tune mechanosensitivity. In epithelial tissues, TRPV4 regulates ciliary beating and barrier permeability. In sensory neurons, it mediates mechanical pain and contributes to thermal sensation. In vascular endothelial cells, TRPV4 activation triggers calcium-dependent vasodilation through nitric oxide release, influencing blood pressure regulation and flow adaptation.
The TRPV4 antibody is widely used in physiology, neuroscience, and cardiovascular research to detect TRPV4 expression and localization. Western blot analysis typically identifies a 98 kilodalton band corresponding to TRPV4, while immunofluorescence reveals membrane and cytoplasmic localization in epithelial and neuronal cells. This antibody allows detailed investigation into TRPV4-mediated calcium signaling and mechanical responsiveness across tissues. Dysregulation of TRPV4 function is associated with diseases such as neuropathic pain, pulmonary edema, skeletal dysplasias, and Charcot-Marie-Tooth neuropathy type 2C.
Mechanistically, TRPV4 operates as a polymodal integrator linking mechanical and chemical cues to calcium signaling and gene expression. Mutations that enhance or suppress TRPV4 channel activity disrupt normal ion homeostasis and cellular volume regulation. The TRPV4 antibody enables researchers to assess expression changes under osmotic, mechanical, or thermal stress, supporting mechanistic studies of signal transduction and cellular adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
