U-Blot® CD223 Rabbit Monoclonal Antibody

SKU:BHA21801243
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-CD223 antibody (Rabbit host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation with reactivity to Human, Mouse, Rat.
Clonality Monoclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG, Kappa
Clone PT1497R
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Options selector
Catalog no. Size
WR5255-50 50 uL
WR5255-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target CD223
Antigen
  • Lymphocyte activation gene 3 protein
Alternative names LAG3;FDC;Lymphocyte activation gene 3 protein;LAG-3;Protein FDC;CD223
Gene ID 3902 (Human) | 16768 (Mouse) | 297596 (Rat)
Host Rabbit
Clone PT1497R
Clonality
  • Monoclonal
Isotype
  • IgG
  • Kappa
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
Conjugate
  • Unconjugated
Molecular weight Calculated: 57 kDa | Observed: 60 kDa
Purification Protein A
Cellular localization [Lymphocyte activation gene 3 protein]: Cell membrane ; Single-pass type I membrane protein .; [Secreted lymphocyte activation gene 3 protein]: Secreted . Produced following cleavage of the main chain. .
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WR5255
Main SKU BHA21801243
Antibody

Product Overview

U-Blot® CD223 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against CD223. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target CD223
Host Rabbit
Clonality Monoclonal
Clone PT1497R
Isotype IgG, Kappa
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Purification Protein A
Specificity Endogenous
Molecular Weight Calculated: 57 kDa | Observed: 60 kDa
UniProt / Swiss-Prot P18627 (Human) | Q61790 (Mouse) | Q5BK54 (Rat)

Recommended dilutions: IHC 1:200-1:1000;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200

Immunogen / Antigen

Lymphocyte activation gene 3 protein

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone PT1497R.

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