| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CENPA;Histone H3-like centromeric protein A;Centromere autoantigen A;Centromere protein A;CENP-A |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Centromeric Protein A around the phosphorylation site of Ser7. AA range:1-50 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CENP-A (Phospho Ser7) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CENP-A. Recognizes Phospho. It has been validated for Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | CENP-A |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-CENP-A (S7) Polyclonal Antibody detects endogenous levels of CENP-A protein only when phosphorylated at S7. |
| Molecular Weight | Calculated: 16 kDa |
| UniProt / Swiss-Prot | P49450 (Human) | O35216 (Mouse) |
Recommended dilutions: IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Centromeric Protein A around the phosphorylation site of Ser7. AA range:1-50
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]
Safety & Handling
This antibody is validated for Immunofluorescence, ELISA. Recommended dilutions: IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
